Acid Hemoglobins Electrophoresis Kit

Ref: SRE647K
Ref: SRE647K
Ref: SRE605K
Ref: SRE605K

SRE605K 13 130

The Acid Hemoglobin Electrophoresis kits are intended for a qualitative test for the identification of both normal and abnormal or variant hemoglobins, and to confirm the identity of clinically relevant hemoglobins such as A, F, S and C. The Acid Hemoglobin test kit employs agarose gel at acid pH and is for in vitro diagnostic use. The kits have been designed for use with the fully automated instruments Easy Interlab G26 & Pretty Interlab.


Reagent Preparation:

All reagents ready to use.

Sample Preparation:

After the red blood cells (RBC) are washed, they are lysed as follows: 50 μl of packed washed RBC + 450 μl of lysing solution.

Sample Storage & Stability:

Whole blood: 1 week at 2 to 8°C

Hemolysate: 12 hours at 2 to 8°C

Synthesis of hemoglobin is under genetic control and the presence of abnormal hemoglobins is associated with functional, physical and morphological abnormalities of the red cells. Hemoglobins are composed of polypeptide chains of globin and iron protoporphyrin heme groups.

Normal hemoglobins have similar structures with a molecular weight of about 67,000 daltons and consist of 2 pairs of globin chains each associated with one molecule of heme.

Hemoglobin A polypeptide chains 2 α and 2 ß
Hemoglobin A2 polypeptide chains 2 α and 2 δ
Hemoglobin F polypeptide chains 2 α and 2 γ

Normal adult hemoglobins are HbA (98% of total hemoglobin) and small amounts of HbA2 and HbF. Substitution of aminoacids in the polypeptide chains sequence leads to the formation of abnormal hemoglobin variants. At present over 600 structural hemoglobins variants are known: HbS, HbC, HbE, HbD, HbG, HbH, HbI, Hb Lepore, etc.

Hemoglobinopathies are a group of diseases caused by the presence of abnormal hemoglobins.

About 60% of abnormal hemoglobins have a sufficiently altered charge distribution to be identified by electrophoresis. The variants most frequently observed are HbS and HbC.

Concomitant presence of HbA and another variant (HbS, HbC, HbE, etc.) is referred to as heterozygous hemoglobinopathy AS, AC and AE respectively.

The presence of only one single type of hemoglobin variant is called homozygous hemoglobinopathy, like HbS or HbC.

These abnormal variants are genetically transmissible. Homozygous hemoglobinopathies may cause serious clinical effects. Acid hemoglobin electrophoresis on agarose allows confirmation of the presence of HbS or HbC already detected by Alkaline Hemoglobins electrophoresis.