10 to 15 percent of couples, despite trying to have a baby, are unable to have children from their gametes and are known as infertile couples, which accounts for half of the infertility cases due to the male factor.

The majority of male infertility is associated with impaired sperm parameters. Therefore, these individuals are candidates for assisted reproductive methods such as IVF and ICSI and may have repeatedly experienced these treatments unsuccessfully.

One of the most important factors in the success of IVF is the health of DNA and sperm chromatin. Various studies have shown that the lower the quality of the main sperm parameters, the lower the sperm DNA health is. considering The fact that damaged  DNA and sperm chromatin may lead to failure of  complicated and expensive treatment such as IVF , so it is necessary to evaluate the rate of sperm DNA breakage (SDF) before choosing the appropriate treatment for couples with recurrent treatment failure. In many cases, identification of sperm DNA damage and choosing appropriate treatment and interference method will  enhance sperm quality.

In this experiment, in a microgel substrate  under an acidic media, chromatin and sperm DNA is denatured and in the next step, by removing chromatin proteins, the DNA strands are distributed as far as possible around the sperm head which after staining  are visible as  halo  around the sperm head. However, the breakdown of sperm DNA leads to lack of  the DNA strands around head and consequently  the lack of aura or very small halo around the sperm head.